• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
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  • 优先权
    • 专利摘要:
    A method for the detection, identification and enumeration of microorganisms in a porous support impregnated dry throughout its thickness by a dehydrated reaction medium, device for implementing said method and use of said device.
    公开号:FR3016169A1
    申请号:FR1450149
    申请日:2014-01-09
    公开日:2015-07-10
    发明作者:Marie-Pierre Montet；Christine Rozand
    申请人:Biomerieux SA；
    IPC主号:
    专利说明:

    [0001] The present invention relates generally to the field of microbiological analysis. DETAILED DESCRIPTION OF THE INVENTION The present invention relates generally to the field of microbiological analysis. More particularly, it relates to a method for detecting, identifying and / or enumerating microorganisms in a porous support impregnated dry throughout its thickness by a dehydrated reaction medium.
    [0002] In the fields of clinical diagnosis and microbiological industrial control of food, pharmaceutical or cosmetics, gelled culture media in Petri dishes, most often agar plates, have been an indispensable tool for detection and identification since the end of the 19th century. pathogenic microorganisms.
    [0003] Several products have been proposed commercially to replace a culture medium in a Petri dish. One of them, the PetrifilmTM system, which contains rehydratable nutrients, is widely used. Another system developed by the company Nissui Pharmaceutical, the Compact DryTM, also consists of a dehydrated medium.
    [0004] These culture media have the advantage of remaining longer than a ready-to-use agar culture medium. They can also, as petrifilmTM present a small footprint and thus use a small incubation space. Thus, there are schematically two ways to obtain a rehydratable culture medium: The first is to bring the culture medium in a liquid form into the support and then to dry the assembly. The second consists in adhering the culture medium in dehydrated form to a support, in order to immediately obtain a rehydratable culture medium.
    [0005] The first method, namely the obtaining of rehydratable nutrient media manufactured by including a phase of wet impregnation of nutrients, has been the subject of several patent applications. Thus, patent application CN102337324 describes a process in which the nutrient broth is mixed with a chemical component whose evaporation is rapid. More recently, patent application US20130089887 describes a support, namely a thin membrane impregnated with chromogenic and / or fluorogenic substrates, brought into contact with an agar medium.
    [0006] However, this method is at least more water, energy and space consuming than the second method and moreover it can negatively impact the shelf life of the rehydratable culture medium. Indeed, the suspension of certain fragile products, such as enzymes, enzymatic or metabolic substrates, antibiotics, can severely impact their overall stability. The heating required for drying the culture medium may also denature and render ineffective the temperature-sensitive components of the reaction medium. This method in the aqueous phase does not allow either to control and vary the location of the reaction medium and / or the various additives necessary for bacterial revelation.
    [0007] In order to overcome the drawbacks of the culture media obtained by this method, the second method proposes to deposit directly on the support the nutritive powder without prior phase of dissolution of the latter. Thus, 3M society offers a dehydrated nutrient medium stuck and deposited on a film without going through a prior phase of solubilization of the medium. This device consists of two parts, a lower film and an upper film covered on the surface with certain components of the dehydrated culture medium. At the time of analysis, the sample is deposited between these two films. This device and the associated detection method have several disadvantages.
    [0008] First, this device requires for its manufacture a dehydrated nutrient adhesion step on the films that had to be previously glued. Then, the culture medium can not de facto form a three-dimensional structure whose height can be varied, and the concentration by strata since it is glued to a film. Only a small surface layer of medium is available. The volume of the liquid sample required for the microbiological analysis can not then exceed 1 or 2 ml, which impacts the detection sensitivity threshold. Then, the packaging of petrifilmTM requires the joint manufacture of the lower film and the upper film. It also does not allow to have on the same device several media different cultures. In addition, this device is limited in its applications and can not, for example, be used as swabs or dressings. And finally, PetrifilmTM requires the help of an outside operator bringing the aqueous sample.
    [0009] On the other hand, in a prior patent application FR1257047, the Applicant proposes a method of isolation from a sample to be analyzed, on a rehydratable culture medium in situ, which makes it possible to obtain isolated colonies. This rehydratable medium is covered with a membrane allowing the realization of the isolation of the colonies. Thus the culture medium remains sterile and colonies grow on the membrane just above it. The isolation of colonies on an agar medium or not, is sometimes seen as a constraint and is often incompatible with experiments carried out outside the laboratory and / or with people with little knowledge and know-how in microbiology. In view of the sum of the problems developed above, the present invention proposes a new method for the detection, identification and enumeration of microorganisms that can be included in a sample.
    [0010] Thus, an object of the present invention is to provide a device comprising a dehydrated medium improving the detection sensitivity. Another object of the present invention is to provide a method for detecting, identifying and enumerating microorganisms without resorting to isolation. Another object of the present invention is to provide a device and a method allowing a multi-detection, and thus obtain from the same sample to be analyzed and without making isolation, isolated cultures, identifiable, countable on different reaction media The invention also aims to provide a device and a particularly flexible process. The reaction medium may be a more or less complex chromogenic medium for example, or very simple, ie containing only a limited number of substrates (antibiotics, metabolic substrates, etc.). The device and method may also have a variety of use patterns such as a swab or absorbent revealing microbial contaminations in dressings, diapers, food packaging. Another object of the present invention is to provide a device whose use is possible by people with little know-how in microbiology. Thus the device can be rehydrated at one time by the operator at the time of analysis of the sample. Rehydration can also be carried out in situ without the aid of the operator if, in particular, the sample to be tested is placed near the rehydratable reaction medium while allowing it to be progressively rehydrated. The sample to be analyzed may for example be an exudative wound or a piece of meat and produce a liquid likely to contain the microorganisms to be detected. Another object of the present invention is to provide a device which itself serves to collect the sample such as a swab.
    [0011] An object of the present invention is to provide a device whose production and marketing are facilitated by the fact that the porous support impregnated dry by a reaction medium is produced independently of its packaging.
    [0012] Another object of the present invention is to provide a device in which a concentration gradient of the reaction substrates is carried out, thus making it possible to limit the quantity of these substrates and to limit the production cost of the device. Other objectives will appear on reading of this application.
    [0013] The present invention therefore aims to achieve all or part of the aforementioned objectives.
    [0014] Accordingly, the present invention relates to a method for detecting and / or identifying and / or enumerating at least one target microorganism in a sample that may contain it, comprising the steps of: (a) providing a device for detecting and / or identifying and / or enumerating microorganisms comprising a porous support dry-impregnated throughout its thickness by a dehydrated reaction medium, (b) contacting a sample with the porous support, (c) incubating the device, (d) detecting and / or identifying and / or listing the microorganism colony (s) within the porous medium, when the microorganisms sought are present in the sample. According to the invention, the device is impregnated dry throughout its thickness by a dehydrated reaction medium. The incorporation of pulverulent materials into porous supports can be carried out according to at least four techniques: - use of a vacuum pump as described in US Pat. No. 5,213,843; - The mechanical vibration of the porous support itself on which the powder was deposited by any vibrating system, shaking to penetrate more or less deeply the powder; - the use of an electrostatic field; - Ultrasonic vibration setting, simultaneously with the application of the powder, using an Ultra Sound generator that vibrates a sonotrode as described in the patent application FR 2866578. When the porous product scrolls under the sonotrode, the action of it causes the powder particles to vibrate, and these then penetrate into the cavities of the porous body. Preferably, the method of manufacturing the porous support dry impregnated throughout its thickness comprises a step of vibrating the powder particles by an electric field. Preferably, this electric field is alternative. Patent EP 1.028.836 describes the impregnation of textiles (non-woven fabrics, fabrics, etc.) by applying an alternating electric field between two electrode systems between which the powder-coated textile is located. Electrically charged powder particles vibrate at the frequency of the alternating field. Surprisingly, this technique can be used to dry impregnate a porous support with a dehydrated reaction medium. The displacements of the particles thus allow their penetration into the porosities of the support. The particles penetrated deeply into the porous support throughout the thickness of the support. Thus, the medium impregnated support areas are impregnated throughout the thickness of the support. Certain areas defined laterally on the support may nevertheless be free of any medium such as for example the periphery of the support.
    [0015] The degree of impregnation of the particles in the thickness of the support can be controlled, for example homogeneous, localized or in gradient, depending on the characteristics of the materials present (supports and powders) but also the characteristics of the process involved (intensity electric field, processing time, frequency ....). The support can be impregnated sequentially in time which can allow better impregnation. Thus, the gelling agent may be impregnated prior to the impregnation of the reaction medium. The dry impregnation of a dehydrated medium throughout the thickness of a porous support does not require the use of water and allows a control of the location of the particles. In addition, it allows flexibility in the thickness of the impregnated layer by allowing to define different areas in the thickness of quantity and different natures. Advantageously, a concentration gradient of the reaction substrates is carried out in the porous support, thus making it possible to limit the quantity of these substrates and to limit the production cost of the device. It may also be chosen to have a porous support comprising a homogeneously distributed type of substrate and another type of substrate distributed in gradient. Advantageously, the support is impregnated throughout its thickness by a nutrient medium and superficially by chromogenic substrates.
    [0016] Thus, the porous support may comprise different reaction media. These reaction media are located in different areas of the support. These zones may correspond to vertical zones and therefore to the thickness of the support and / or correspond to horizontal zones of the support. In practice, several parameters can influence the implementation of this method as mainly: the texture of the network of fibers or filaments, or in general of the porous support used; the physicochemical characteristics of the powders, such as the nature or the particle size of the powder; the duration of treatment, the intensity of the electric field and the frequency of the electric field; It will therefore be necessary to adapt these parameters to allow a satisfactory dry impregnation of the reaction medium in the porous support. Preferably, the amount of reaction medium impregnated in the porous support is between 0.10 mg / cm3 and 0.1 g / cm3, preferably between 0.01 g / cm3 and 0.09 g / cm3, more preferably between 0.03 g / cm3 and 0.06 g / cm3. cm3. Preferably, when the reaction medium comprises a culture medium and optionally a revelation medium, the amount of impregnated reaction medium is between 0.01 g / cm 3 and 0.09 g / cm 3, more preferentially between 0.03 g / cm 3 g and 0.06 g. g / cm3. Preferably, when the reaction medium comprises a development medium without culture medium, the amount of impregnated reaction medium is much lower and is between 0.10mg / cm3 and 10mg / cm3.
    [0017] According to the invention, the porous support is brought into contact with the sample. In one embodiment of the invention, the sample is aqueous and will allow rehydration of the reaction medium included in the porous support.
    [0018] According to another embodiment, an appropriate volume of liquid is added to the sample and / or the porous support in order to rehydrate the reaction medium, when the sample is not aqueous or insufficiently aqueous.
    [0019] In practice, those skilled in the art will choose the appropriate volume of liquid or aqueous sample depending on its viscosity, the diameter of the porous support, in order to rehydrate the medium and allow the growth of microorganisms.
    [0020] Advantageously, the rehydration of the porous support requires a volume of liquid or aqueous sample greater than 2m1, preferably greater than 3m1, more preferably greater than 4m1, which improves the detection sensitivity when the microorganisms are in low concentration in the sample.
    [0021] According to the present invention, the sample may comprise a preliminary step of preparation, concentration or dilution of the sample. According to the invention, the rehydration of the support can be carried out with or without the intervention of an operator.
    [0022] The aqueous sample can be added manually using a pipette or automatically into the device. It can also be contained in at least one tank integrated in the device and / or channels allowing rehydration of the porous support. It then spreads in the support by simply pressing the tank. Advantageously, the sample is brought into contact with the porous support by placing it under the porous support. Thus, the rehydration takes place by the lower and / or lateral part, preferably by the lower part. This procedure allows a homogeneous hydration of all the porous support and avoids, in particular, the nutrients and / or substrates to be entrained by the liquid or the aqueous sample, in the lower part of the device. Advantageously, this procedure allows the method according to the invention to be carried out in space by solving the problem related to the absence of gravity of the sample and / or the liquid. In one embodiment, there is no human intervention and the aqueous sample comes directly from a producing zone of the liquid to be tested. It may be for example an exudative wound, food releasing liquids during storage. The sample, by its nature, will release some of its constituent liquids that go over time soak the porous support. The producing area of the test sample may also be a perineal area of humans or animals excreting urine. The porous support is then placed near this zone and is progressively impregnated with the aqueous sample produced. In another embodiment, the sample is brought into contact with the porous support by taking the sample with the porous support. The porous support is then used as a swab and the operator will place the latter in a tube containing the appropriate amount of liquid if the sample is not aqueous or insufficiently aqueous. The device is then incubated in situ (in the case of dressings, the diaper, etc.) or in an incubator for a period of time sufficient to allow the detection of microbial colonies within the porous support. According to a preferred embodiment, the method according to the invention is a detection method that can be implemented by visual or optical reading of the porous support.
    [0023] The invention also relates to a device comprising a porous support impregnated dry throughout its thickness by a dehydrated reaction medium for revealing colonies of microorganisms within said support, said porous support being calendered. The porous support was impregnated dry throughout its thickness, that is to say deep down in a region of the support defined laterally that is to say horizontally. The porous support has undergone a calendering operation. The calendering, by the pressure and the heating temperature generated, allows retention and stable maintenance over time of the dehydrated reaction medium in the porous support, ensuring the retention of the various elements such as nutrients in the porous support. It also makes it possible to obtain a rigorously smooth and flat top surface of the porous support. Preferentially, the calendering is carried out at a temperature greater than ambient temperature, preferably at a temperature of between 30 ° C. and 60 ° C. A temperature below 60 ° C makes it possible not to denature thermolabile compounds.
    [0024] The calendering allows, in addition to accelerating the rehydration of the porous support relative to non-calendered porous support, a compression of the fibers constituting the porous support. This compression, associated with the presence of the dehydrated medium within the porous support allows simultaneous rehydration in all areas of the support placed horizontally and thus preserves the chosen distribution (homogeneous or gradient) of the substances. The calendering thus makes it possible to maintain the reaction medium inside the support and its easy handling. Preferably, the device comprises a protective upper layer. The protective layer is disposed on the porous support and no other layer is disposed between the porous support and the protective layer. Preferably the protective layer is placed directly on the porous support. The protective layer can be translucent or transparent allowing the visibility of the colonies through this layer. It also prevents contamination during incubation. It is impervious to bacteria and limits the loss of water vapor. Indeed, the device is incubated for a time and at a predetermined temperature allowing the growth of microorganisms regardless of ambient humidity conditions. Thus, the nature of the upper layer is chosen so as to allow the gas exchange necessary for the growth of microorganisms while allowing local hydration.
    [0025] Preferably, the device comprises a receptacle for containing the aqueous sample and / or the liquid. Preferably, the receptacle also comprises the porous support which is then preferentially rehydrated by its lower part. Advantageously, the device comprises a lower layer impermeable to water.
    [0026] Preferably this lower layer is rigid, allowing better grip of the device. It is made from compounds such as polyester, polypropylenes, polystyrene. Preferably, it is made from cellulose. It can be cardboard or paper associated with a waterproof film. It may contain thermoformed channels which will be used for the proper rehydration of the porous support.
    [0027] Advantageously, the various layers of the device are made from recyclable materials. According to a particular embodiment of the invention, the lower layer is translucent or transparent. According to a particular embodiment of the invention, the device also comprises an identification code such as barcodes or RFID tags.
    [0028] According to the present invention, it is also possible to combine within the same device different reaction media arranged next to each other and which will be rehydrated simultaneously with the same sample to be tested. In one embodiment, the device comprises a rod at the end of which the porous support, impregnated dry by a dehydrated reaction medium, is fixed. Fixing can be performed by any means known to those skilled in the art such as gluing or heat sealing. The porous support attached to a rod can thus act as a swab. Advantageously, the device also comprises a transparent waterproof tube able to collect the swab and a hermetic plug of the tube. After performing the sampling by the swab, it is placed in the tube which contains the water necessary for the rehydration of the porous support placed at its apical part. The tube is then plugged and incubated.
    [0029] In another embodiment, the porous carrier is embedded in a bandage, bandage, diaper, or food package. Preferably, the device comprises, under the porous support, a porous lower layer which is brought into contact with the sample to be analyzed. Even more preferentially, the device also comprises a transparent impermeable upper layer over at least a portion.
    [0030] The invention also relates to the use of a device according to the invention for detecting and / or identifying and / or enumerating at least one target microorganism in a sample likely to contain it. Advantageously, the invention relates to the use of a device comprising a rod at the end of which said porous support is fixed, as a swab. In another embodiment, the invention relates to the use of a device according to the invention as a dressing. According to another embodiment, the invention also relates to the use of a device according to the invention as a panty layer.
    [0031] In yet another embodiment, the invention relates to the use of a device according to the invention as a food packaging. A sample is understood to mean a small or small quantity separated from an entity by a subtractive act, usually called sampling, for analysis purposes.
    [0032] The sample may be of biological, human, animal, plant or environmental origin. It may relate to a product during an industrial process or a finished product, for example food. It may therefore correspond to a biological fluid sample (whole blood, serum, plasma, urine, cerebrospinal fluid, organic secretion), a tissue sample or isolated cells. It may be of industrial origin, or, according to a non-exhaustive list an air sample, a water sample, a sample taken on a surface, a part or a product being processed or manufactured, a product of food origin. Examples of food-based samples include, but are not limited to, a sample of dairy products (yogurts, cheeses, etc.), meat, fish, eggs, fruits, vegetables, water, drink (milk, fruit juice, soda, etc.) and the constituent products or annexes of the finished product. A food sample may finally be derived from a feed intended for animals, such as in particular animal meal. This sample may undergo prior to its analysis a preparation type enrichment, extraction, concentration, purification, according to methods known to those skilled in the art.
    [0033] For the purposes of the present invention, the term microorganism covers gram-positive or gram-negative bacteria, yeasts, molds, amoebae and, more generally, unicellular organisms, invisible to the naked eye, which can be handled and multiplied in the laboratory. According to a preferred embodiment of the invention, the microorganism is a bacterium, gram negative or positive, or a yeast.
    [0034] As Gram-positive bacteria, mention may be made of the following genera: Enterococcus, Streptococcus, Lactobacillus, Bifidobacterium, Staphylococcus, Bacillus, Listeria, Clostridium, Mycobacteria, Nocardia, Corynebacteria, Micrococcus and Deinococcus. Gram-negative bacteria include the following genera: Salmonella, Escherichia coli and Pseudomonas. Yeasts that may be mentioned include yeasts of the following genera: Candida, Cryptococcus, Saccharomyces and Trichosporon. As molds, mention may be made of the following types of mold: Aspergillus, Penicillium, Cladosporium Porous support is understood to mean a volume with suitable porosity, in the form of fabrics or nonwoven having a fibrous or filamentous network, of foam open porosity. It is a three-dimensional support in which the particles of the reaction medium have penetrated into a given zone of the porous support throughout its thickness. The support may be based on various absorbent compounds with very high water retention capacity such as viscose, rayon, cotton, natural or chemically modified cellulose fibers such as carboxymethyl cellulose, absorbent or super absorbent chemical polymers. absorbents such as polyacrylate salts, acrylate / acrylamide copolymer. Preferably, the porous support will have a surface density of between 50 g / m 2 and 150 g / m 2, preferably between 90 g / m 2 and 110 g / m 2. Preferably, the porous support has a thickness of 0.5 to 2 mm, preferably 0.8 to 1.5 mm. The porous support is able to retain a volume of water greater than 2m1, preferably greater than 3m1.
    [0035] By reaction medium means a medium comprising all the elements necessary for the survival and / or growth of microorganisms. This reaction medium can either serve only as a revelation medium, or serve as a culture medium and revelation. In the first case, the culture of microorganisms takes place beforehand, and in the second case, the reaction medium also constitutes the culture medium. Thus, the reaction medium of the device according to the invention is a revelation medium and / or a culture medium. The term "revealing medium" means any medium containing a molecule capable of coupling with the microorganisms or the binding partners of said microorganisms and which, by virtue of their transduction properties (fluorescence, coloring, radioactivity, etc.), to reveal the presence of said microorganisms. This revelation of the presence of the target microorganisms may be obtained in particular by visualization (with the naked eye) or optical reading of a coloration or fluorescence on all or part of the support.
    [0036] By culture medium is meant a medium comprising all the elements necessary for the survival and / or growth of microorganisms. In practice, a person skilled in the art will choose the culture medium according to the target microorganisms, according to criteria that are well known to those skilled in the art. The reaction medium according to the invention may contain any additives such as for example: peptones, one or more growth factors, carbohydrates, one or more selective agents, buffers, dyes, one or more gelling agents, hydrogels , viscous agent, etc .. Preferably, the reaction medium of the device according to the invention comprises at least one gelling agent whose quantity is between 1 mg / cm 2 and 2 mg / cm 2. The gelling agent may be chosen from gelling agents well known to those skilled in the art such as agar, agarose, poloxamers, guar gum, xanthan. Thus, the porous support and the gelling agents make it possible to limit the diffusion of substrates and microorganisms and participate in the formation of isolated microbial colonies.
    [0037] By "at least one target microorganism" is meant, within the meaning of the present invention, at least one microorganism that one wishes to detect and / or identify and / or enumerate.
    [0038] For the purposes of the present invention, the definition of "detection sensitivity" is identical to that commonly accepted in the state of the art, namely the ability to give a positive result (appearance of a colored and / or fluorescent reaction). ) when the target bacterial strain is present in the sample. 15
    权利要求:
    Claims (10)
    [0001]
    CLAIMS1 A method for the detection and / or identification and / or enumeration of at least one target microorganism in a sample capable of containing it, comprising the following steps: (a) providing a device for detection and / or identification and / or enumerating microorganisms comprising a porous support dry-impregnated throughout its thickness by a dehydrated reaction medium, (b) contacting a sample with the porous support, (c) incubating the device, (d) detecting and / or identify and / or enumerate the colony or colonies of microorganisms within the porous support, when the microorganisms sought are present in the sample.
    [0002]
    2 Process according to claim 1 comprising a preliminary step of preparation, dilution or concentration of the sample.
    [0003]
    The method of any one of claims 1 or 2, wherein step b) is performed by placing the sample below the porous support.
    [0004]
    4. The method according to claim 1, wherein step b) is carried out by taking the sample using the porous support.
    [0005]
    A process according to any one of the preceding claims, wherein when the sample is not aqueous or insufficiently aqueous, an appropriate volume of liquid is added to the sample and / or porous carrier to rehydrate the medium. reaction.
    [0006]
    6 Device comprising a porous support impregnated dry throughout its thickness by a dehydrated reaction medium for revealing colonies of microorganisms within said support, said porous support being calendered.
    [0007]
    7 Apparatus according to claim 6 wherein the reaction medium is a developing medium and / or a culture medium.
    [0008]
    8 Apparatus according to any one of claims 6 or 7 wherein the reaction medium comprises at least one gelling agent whose amount is between 1 mg / cm 2 and 2 mg / cm 2.
    [0009]
    9 Apparatus according to any one of claims 6 to 8 wherein the amount of reaction medium impregnated in the porous support is between 0.10mg / cm3 and 10 0.1g / cm3, preferably between 0.01 g / cm3 and 0.09 g / cm3.
    [0010]
    Apparatus comprising a plurality of reaction media according to claim 6 to 9. The device of any one of claims 6 to 10 wherein the porous carrier is embedded in a dressing, bandage, diaper or food package. 12 Apparatus according to any one of claims 6 to 10 comprising a rod 20 at the end of which said porous support is fixed. Use of a device according to any of claims 6 to 12 for detecting and / or identifying and / or enumerating at least one target microorganism in a sample capable of containing it. The use of a device according to claim 11 integrated in a dressing. Use of a device according to claim 11 integrated in a diaper. Use of a device according to claim 11 integrated in a food package. Use of a device according to claim 12 integrated in a swab. 10 15 20 25 30
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    同族专利:
    公开号 | 公开日
    US20160326564A1|2016-11-10|
    WO2015104501A1|2015-07-16|
    CN105899653A|2016-08-24|
    FR3016169B1|2017-12-15|
    EP3092297A1|2016-11-16|
    US10144947B2|2018-12-04|
    CN105899653B|2019-08-13|
    EP3092297B1|2019-06-19|
    ES2744180T3|2020-02-24|
    BR112016015647B1|2021-07-20|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US20010012537A1|1999-07-30|2001-08-09|Anderson Norman G.|Dry deposition of materials for microarrays using matrix displacement|
    WO2002002245A2|2000-06-29|2002-01-10|Johnson & Johnson Consumer Companies, Inc.|Electrostatic impregnation of powders on substrates|
    WO2003093819A1|2002-05-03|2003-11-13|Kimberly-Clark Worldwide, Inc.|Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices|
    WO2005038123A1|2003-10-21|2005-04-28|Materials Technics Holding Sa|Method and device for impregnating a fibrous web with a powder using an alternating electrostatic field|
    WO2005061013A1|2003-12-05|2005-07-07|Kimberly-Clark Worldwide, Inc.|Personal care products with visual indicator of vaginitis|
    WO2009082667A1|2007-12-21|2009-07-02|3M Innovative Properties Company|Microbiological systems and methods of fluid sample analysis|
    WO2010001043A1|2008-07-02|2010-01-07|Fibroline France|Device and method for impregnating a porous material with powder|
    US20130089887A1|2011-10-10|2013-04-11|Sergey Gazenko|Method for Rapid Detection and Identification of micro-colonies using impregnated porous material|
    FR1257047A|1960-02-13|1961-03-31|Improvement in artificial snow packaged in aerosols|
    US3937655A|1974-04-16|1976-02-10|Eli Lilly And Company|Method for preparing stable β-lactam-type-antibiotic susceptibility test discs|
    US5213843A|1991-08-05|1993-05-25|The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration|Vacuum powder injector and method of impregnating fiber with powder|
    EP0914916A1|1997-11-04|1999-05-12|Materials Technics Société Anonyme Holding|Method for producing a composite material|
    FR2866578B1|2004-02-23|2007-01-12|Agro Fibres Technologies Plast|NEW POROUS MEDIA: ALVEOLAR, FOAMS, FABRICS, FELTS, AND ANALOGS, IMPROVED BY INCORPORATED ORGANIC OR MINERAL POWDERS, ULTRA-SOUND INCORPORATION METHOD, ADAPTED PROBE AND APPLICATIONS THEREOF|
    TW200712472A|2005-08-02|2007-04-01|3M Innovative Properties Co|Apparatus and method for collecting a sample of material|
    CN101319248A|2007-06-08|2008-12-10|广州绿洲生化科技有限公司|Method for directly preparing microorganism testing slice with commercial product culture medium dry powder|
    US8828682B2|2008-12-09|2014-09-09|3M Innovative Properties Company|Methods and articles for detecting hemolytic microorganisms|
    CN101497914A|2008-12-30|2009-08-05|广东省微生物研究所|Test chip for fast detecting microorganism, preparation and use thereof|
    CN102337324A|2011-10-24|2012-02-01|西南大学|Preparation method of adsorption pad type culture medium for detecting microorganisms|FR2993573B1|2012-07-20|2016-02-26|Biomerieux Sa|METHOD FOR ISOLATING MICROORGANISMS ON A CULTURE MEDIUM AND DEVICE THEREFOR|
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    优先权:
    申请号 | 申请日 | 专利标题
    FR1450149A|FR3016169B1|2014-01-09|2014-01-09|METHOD FOR DETECTION, IDENTIFICATION AND DETECTION OF MICROORGANISMS IN A POROUS SUPPORT DRY IMPREGNATED BY A REACTIONAL MEDIUM DEHYDRATE|FR1450149A| FR3016169B1|2014-01-09|2014-01-09|METHOD FOR DETECTION, IDENTIFICATION AND DETECTION OF MICROORGANISMS IN A POROUS SUPPORT DRY IMPREGNATED BY A REACTIONAL MEDIUM DEHYDRATE|
    ES15702538T| ES2744180T3|2014-01-09|2015-01-08|Detection, identification and enumeration procedure of microorganisms in a dry impregnated porous support with a dehydrated reaction medium|
    BR112016015647-1A| BR112016015647B1|2014-01-09|2015-01-08|METHOD FOR DETECTION AND/OR IDENTIFICATION AND/OR Enumeration OF AT LEAST ONE TARGET MICROORGANISM IN A SAMPLE LIKELY TO CONTAIN IT, A DEVICE INCLUDING A POROUS SUPPORT AND ITS USES|
    CN201580003911.2A| CN105899653B|2014-01-09|2015-01-08|The method of detection, identification and enumeration of micro organisms in the porous holder of dehydration culture medium dry method infiltration|
    PCT/FR2015/050035| WO2015104501A1|2014-01-09|2015-01-08|Method for detecting, identifying and enumerating micro-organisms in a porous support dry-impregnated with a dehydrated reaction medium|
    EP15702538.8A| EP3092297B1|2014-01-09|2015-01-08|Method for detecting, identifying and enumerating micro-organisms in a porous support dry-impregnated with a dehydrated reaction medium|
    US15/109,025| US10144947B2|2014-01-09|2015-01-08|Method for detecting, identifying and enumerating micro-organisms in a porous support dry-impregnated with a dehydrated reaction medium|
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